To unassign a macro from a pin:
In the Hierarchy window (Logical or Physical tab), select the macro to unassign.
From the Logic menu, choose Unassign From Location. This command is available only if the macro has been assigned to a location.
To unassign a macro from a region:
In the Hierarchy window (Logical or Physical tab), select the macro to unassign.
From the Logic menu, choose Unassign From Region. This command is available only if the macro has been assigned to a region.
Tip: You can also right-click the macro, and choose Unassign From Region or Unassign From Location.
To unassign all I/Os from a location, choose Unassign All From Location from the Logic menu. To unassign all I/Os from the selected region, choose Unassign All From Region from the Logic menu.